Registration Statement - Additional Securities (Form S-3MEF)

As filed with the Securities and Exchange Commission on May 4, 2026.

Registration No. 333-   

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

Washington, D.C. 20549

FORM S-3

REGISTRATION STATEMENT

UNDER

THE SECURITIES ACT OF 1933

Aura Biosciences, Inc.

(Exact name of registrant as specified in its charter)

Delaware		32-0271970
(State or other jurisdiction of incorporation or organization)		(I.R.S. Employer Identification Number)

80 Guest Street

Boston, MA 02135

(617) 500-8864

(Address, including zip code, and telephone number, including area code, of registrant's principal executive offices)

Natalie Holles

Chief Executive Officer and President

Aura Biosciences, Inc. 80 Guest Street

Boston, MA 02135

(617) 500-8864

(Name, address, including zip code, and telephone number, including area code, of agent for service)

With copies to:

Danielle Lauzon, Esq.

Stephanie A. Richards, Esq.

Goodwin Procter LLP

100 Northern Avenue

Boston, MA 02210

Telephone: (617) 570-1000

Approximate date of commencement of proposed sale to the public: From time to time after this registration statement becomes effective.

If the only securities being registered on this Form are being offered pursuant to dividend or interest reinvestment plans, please check the following box. ☐

If any of the securities being registered on this Form are to be offered on a delayed or continuous basis pursuant to Rule 415 under the Securities Act of 1933, as amended, other than securities offered only in connection with dividend or interest reinvestment plans, check the following box: ☒

If this Form is filed to register additional securities for an offering pursuant to Rule 462(b) under the Securities Act, please check the following box and list the Securities Act registration statement number of the earlier effective registration statement for the same offering. ☒ 333-278253

If this Form is a post-effective amendment filed pursuant to Rule 462(c) under the Securities Act, check the following box and list the Securities Act registration statement number of the earlier effective registration statement for the same offering. ☐

If this Form is a registration statement pursuant to General Instruction I.D. or a post-effective amendment thereto that shall become effective upon filing with the Commission pursuant to Rule 462(e) under the Securities Act, check the following box. ☐

If this Form is a post-effective amendment to a registration statement filed pursuant to General Instruction I.D. filed to register additional securities or additional classes of securities pursuant to Rule 413(b) under the Securities Act, check the following box. ☐

Indicate by check mark whether the registrant is a large accelerated filer, an accelerated filer, a non-accelerated filer, a smaller reporting company, or an emerging growth company. See the definitions of "large accelerated filer," "accelerated filer," "smaller reporting company," and "emerging growth company" in Rule 12b-2 of the Exchange Act.

Large accelerated filer		☐		Accelerated filer		☐
Non-accelerated filer		☒		Smaller reporting company		☒
				Emerging growth company		☒

If an emerging growth company, indicate by check mark if the registrant has elected not to use the extended transition period for complying with any new or revised financial accounting standards provided pursuant to Section 7(a)(2)(B) of the Securities Act. ☐

The Registration Statement shall become effective upon filing in accordance with Rule 462(b) under the Securities Act of 1933, as amended.

EXPLANATORY NOTE AND INCORPORATION OF

CERTAIN INFORMATION BY REFERENCE

Pursuant to Rule 462(b) under the Securities Act of 1933, as amended, Aura Biosciences, Inc. (the "Registrant") is filing this Registration Statement on Form S-3 (this "Registration Statement") with the Securities and Exchange Commission (the "Commission"). This Registration Statement relates to the public offering of securities contemplated by the Registration Statement on Form S-3 (File No. 333-278253) (the "Prior Registration Statement"), which the Registrant initially filed with the Commission on March 27, 2024, and which the Commission declared effective on April 5, 2024.

The Registrant is filing this Registration Statement for the sole purpose of increasing the aggregate amount of securities offered by the Registrant by a proposed aggregate offering price of $49,907,450.00, which includes shares that may be sold by the Registrant in the event the underwriters exercise their option to purchase additional shares of the Registrant's common stock. The additional securities that are being registered for issuance and sale are in an amount and at a price that together represent no more than 20% of the maximum aggregate offering price of unsold securities under the Prior Registration Statement. The information set forth in the Prior Registration Statement and all exhibits to the Prior Registration Statement are hereby incorporated by reference into this Registration Statement.

The required opinion and consents are listed on the Exhibit Index below and filed herewith or incorporated by reference herein.

PART II

INFORMATION NOT REQUIRED IN PROSPECTUS

Item 16.

Exhibits

All exhibits filed with or incorporated by reference in the Registration Statement on Form S-3 (File No. 333-278253) are incorporated by reference into, and shall be deemed a part of, this Registration Statement, and the following additional exhibits are filed herewith, as part of this Registration Statement:

Exhibit No.		Description
5.1		Opinion of Goodwin Procter LLP.
23.1		Consent of Ernst & Young LLP Independent Registered Public Accounting Firm.
23.2		Consent of Goodwin Procter LLP (see Exhibit 5.1).
24.1		Power of Attorney (incorporated by reference to Exhibit 24.1 to the Prior Registration Statement).
107		Filing Fee Table.

SIGNATURES

Pursuant to the requirements of the Securities Act of 1933, the registrant has duly caused this Registration Statement on Form S-3 to be signed on its behalf by the undersigned, thereunto duly authorized, in the City of Boston, Commonwealth of Massachusetts, on the 4th day of May, 2026.

Pursuant to the requirements of the Securities Act of 1933, this registration statement has been signed below by the following persons in the capacities and on the dates indicated.

Signatures		Title		Date
/s/ Natalie Holles Natalie Holles		Chief Executive Officer and President (Principal Executive Officer)		May 4, 2026
/s/ Anthony Gibney Anthony Gibney		Chief Financial and Business Officer (Principal Financial Officer)		May 4, 2026
/s/ Amy Elazzouzi Amy Elazzouzi		Senior Vice President, Finance (Principal Accounting Officer)		May 4, 2026
				May 4, 2026
/s/ David Johnson David Johnson		Director		May 4, 2026
/s/ Teresa Bitetti Teresa Bitetti		Director		May 4, 2026
				May 4, 2026
/s/ Giovanni Mariggi Giovanni Mariggi		Director		May 4, 2026
/s/ Antony Mattessich Antony Mattessich		Director		May 4, 2026
				May 4, 2026
/s/ Sapna Srivastava Sapna Srivastava		Director		May 4, 2026
/s/ Karan Takhar Karan Takhar		Director		May 4, 2026