Janus Investment Fund

03/04/2026 | Press release | Distributed by Public on 03/04/2026 05:01

Prospectus by Investment Company (Form 497)

Janus Investment Fund

Janus Henderson Low Duration Multi-Sector Income Fund

Supplement dated March 4, 2026

to Currently Effective Prospectuses

and Statement of Additional Information

Effective immediately, Janus Henderson Low Duration Multi-Sector Income Fund (formerly named Janus Henderson Absolute Return Income Opportunities Fund) (the "Fund") will no longer be offered through the combined Fixed Income Prospectuses dated October 28, 2025, and as further supplemented. Additionally, the Fund will no longer be offered through the combined Statement of Additional Information dated October 28, 2025, and as further supplemented. All references to the Fund are removed from the Prospectuses and Statement of Additional Information, accordingly.

In seeking information about the Fund, existing shareholders and prospective eligible investors should refer to the Prospectuses and Statement of Additional Information for the Fund dated March 4, 2026, which may be updated from time to time.

Please retain this Supplement with your records.

Janus Investment Fund

Janus Henderson Balanced Fund Janus Henderson Global Select Fund
Janus Henderson Contrarian Fund Janus Henderson Global Sustainable Equity Fund
Janus Henderson Enterprise Fund Janus Henderson Global Technology and Innovation Fund
Janus Henderson European Focus Fund Janus Henderson Growth and Income Fund
Janus Henderson Forty Fund Janus Henderson Overseas Fund
Janus Henderson Global Equity Income Fund Janus Henderson Research Fund
Janus Henderson Global Life Sciences Fund Janus Henderson Triton Fund
Janus Henderson Global Real Estate Fund Janus Henderson Venture Fund
Janus Henderson Global Research Fund

(collectively, the "Funds")

Class A Shares

Class C Shares

Supplement dated March 4, 2026

to Currently Effective Prospectuses

Effective March 4, 2026, the following information is added after "Ameriprise Financial" in the Appendix A - Intermediary Sales Charge Waivers and Discounts section of the Funds' prospectuses:

BAIRD

The following information is provided by Robert W. Baird & Co. ("Baird"):

Effective March 4, 2026, shareholders purchasing fund shares through a Baird platform or account will only be eligible for the following sales charge waivers (front-end sales charge waivers and CDSC waivers) and discounts, which may differ from those disclosed elsewhere in this prospectus or the SAI

Front-End Sales Charge Waivers on Investors A-shares Available at Baird

Shares purchased through reinvestment of capital gains distributions and dividend reinvestment when purchasing shares of the same fund

Shares purchased by employees and registered representatives of Baird or its affiliates and their family members as designated by Baird

Shares purchased within 90 days following a redemption from a Janus Henderson Fund, provided (1) the redemption and purchase occur within the purchaser's Baird household and (2) the redeemed shares were subject to a front-end or deferred sales charge (known as rights of reinstatement)

A shareholder in the Fund's Investor C Shares will have their share converted at net asset value to Investor A shares of the same fund if the shares are no longer subject to CDSC and the conversion is in line with the policies and procedures of Baird

Employer-sponsored retirement plans or charitable accounts in a transactional brokerage account at Baird, including 401(k) plans, 457 plans, employer-sponsored 403(b) plans, profit sharing and money purchase pension plans and defined benefit plans. For purposes of this provision, employer-sponsored retirement plans do not include SEP IRAs, Simple IRAs or SAR-SEPs

CDSC Waivers on Investor A and C shares Available at Baird

Shares sold due to death or disability of the shareholder

Shares sold as part of a systematic withdrawal plan as described in the Fund's Prospectus

Shares bought due to returns of excess contributions from an IRA Account

Shares sold as part of a required minimum distribution for IRA and retirement accounts due to the shareholder reaching the qualified age based on applicable Internal Revenue Service regulations

Shares sold to pay Baird fees but only if the transaction is initiated by Baird

Shares acquired through a right of reinstatement

Front-End Sales Charge Discounts Available at Baird: Breakpoints and/or Rights of Accumulations

Breakpoints as described in this prospectus

Rights of accumulations which entitles shareholders to breakpoint discounts will be automatically calculated based on the aggregated holding of Janus Henderson assets held by accounts within the purchaser's household at Baird. Eligible Janus Henderson assets not held at Baird may be included in the rights of accumulations calculation only if the shareholder notifies his or her financial advisor about such assets

Letters of Intent (LOI) allow for breakpoint discounts based on anticipated purchases of Janus Henderson through Baird, over a 13-month period of time

Please retain this Supplement with your records.

2

Janus Investment Fund

Janus Henderson Adaptive Risk Managed U.S. Equity Fund Janus Henderson International Dividend Fund
Janus Henderson Developed World Bond Fund Janus Henderson Mid Cap Value Fund
Janus Henderson Flexible Bond Fund Janus Henderson Multi-Sector Income Fund
Janus Henderson Global Allocation Fund - Conservative Janus Henderson Short Duration Flexible Bond Fund
Janus Henderson Global Allocation Fund - Growth Janus Henderson Small Cap Value Fund
Janus Henderson Global Allocation Fund - Moderate Janus Henderson Small-Mid Cap Value Fund

Janus Henderson High-Yield Fund

(collectively, the "Funds")

Class A Shares

Class C Shares

Supplement dated March 4, 2026

to Currently Effective Prospectuses

Effective March 4, 2026, the following information is added after "Ameriprise Financial" in the Appendix A - Intermediary Sales Charge Waivers and Discounts section or, as applicable, Appendix B - Intermediary Sales Charge Waivers and Discounts section of the Funds' prospectuses:

BAIRD

The following information is provided by Robert W. Baird & Co. ("Baird"):

Effective March 4, 2026, shareholders purchasing fund shares through a Baird platform or account will only be eligible for the following sales charge waivers (front-end sales charge waivers and CDSC waivers) and discounts, which may differ from those disclosed elsewhere in this prospectus or the SAI

Front-End Sales Charge Waivers on Investors A-shares Available at Baird

Shares purchased through reinvestment of capital gains distributions and dividend reinvestment when purchasing shares of the same fund

Shares purchased by employees and registered representatives of Baird or its affiliates and their family members as designated by Baird

Shares purchased within 90 days following a redemption from a Janus Henderson Fund, provided (1) the redemption and purchase occur within the purchaser's Baird household and (2) the redeemed shares were subject to a front-end or deferred sales charge (known as rights of reinstatement)

A shareholder in the Fund's Investor C Shares will have their share converted at net asset value to Investor A shares of the same fund if the shares are no longer subject to CDSC and the conversion is in line with the policies and procedures of Baird

Employer-sponsored retirement plans or charitable accounts in a transactional brokerage account at Baird, including 401(k) plans, 457 plans, employer-sponsored 403(b) plans, profit sharing and money purchase pension plans and defined benefit plans. For purposes of this provision, employer-sponsored retirement plans do not include SEP IRAs, Simple IRAs or SAR-SEPs

CDSC Waivers on Investor A and C shares Available at Baird

Shares sold due to death or disability of the shareholder

Shares sold as part of a systematic withdrawal plan as described in the Fund's Prospectus

Shares bought due to returns of excess contributions from an IRA Account

Shares sold as part of a required minimum distribution for IRA and retirement accounts due to the shareholder reaching the qualified age based on applicable Internal Revenue Service regulations

Shares sold to pay Baird fees but only if the transaction is initiated by Baird

Shares acquired through a right of reinstatement

Front-End Sales Charge Discounts Available at Baird: Breakpoints and/or Rights of Accumulations

Breakpoints as described in this prospectus

Rights of accumulations which entitles shareholders to breakpoint discounts will be automatically calculated based on the aggregated holding of Janus Henderson assets held by accounts within the purchaser's household at Baird. Eligible Janus Henderson assets not held at Baird may be included in the rights of accumulations calculation only if the shareholder notifies his or her financial advisor about such assets

Letters of Intent (LOI) allow for breakpoint discounts based on anticipated purchases of Janus Henderson through Baird, over a 13-month period of time

Please retain this Supplement with your records.

2

Janus Investment Fund

Janus Henderson International Dividend Fund

Supplement dated March 4, 2026

to Currently Effective Prospectuses

Effective immediately, the prospectuses for Janus Henderson International Dividend Fund (the "Fund") are amended as follows:

Combined Share Class Prospectus

1.

Under "Distributions" in the Distribution and Taxes section of the Fund's prospectuses, the following paragraph replaces the corresponding paragraph in its entirety:

Distribution Schedule

Dividends from net investment income for Janus Henderson International Dividend Fund are normally declared and distributed in March, June, September, and December. Dividends from net investment income for each of the other Funds are normally declared and distributed in December. In addition, distributions of net capital gains are normally declared and distributed in December. If necessary, dividends and net capital gains may be distributed at other times as well. The date you receive your distribution may vary depending on how your intermediary processes trades. Please consult your intermediary for details.

Class D Shares Prospectus

2.

Under "Distributions" in the Distribution and Taxes section of the Fund's prospectuses, the following paragraph replaces the corresponding paragraph in its entirety:

Distribution Schedule

Dividends from net investment income for Janus Henderson International Dividend Fund are normally declared and distributed in March, June, September, and December. Dividends from net investment income for each of the other Funds are normally declared and distributed in December. In addition, distributions of net capital gains are normally declared and distributed in December. If necessary, dividends and net capital gains may be distributed at other times as well.

Please retain this Supplement with your records.

Janus Investment Fund

Janus Henderson Adaptive Risk Managed U.S. Equity Fund Janus Henderson Mid Cap Value Fund
Janus Henderson Developed World Bond Fund Janus Henderson Multi-Sector Income Fund
Janus Henderson Flexible Bond Fund Janus Henderson Short Duration Flexible Bond Fund
Janus Henderson High-Yield Fund Janus Henderson Small Cap Value Fund
Janus Henderson International Dividend Fund Janus Henderson Small-Mid Cap Value Fund

(the "Funds")

Class A Shares Class D Shares Class N Shares Class S Shares
Class C Shares Class I Shares Class R Shares Class T Shares

Supplement dated March 4, 2026

to Currently Effective Statement of Additional Information

Effective immediately, the statement of additional information ("SAI") for the Funds is updated as follows:

1.

Under "Janus Henderson Portfolio Management" in the Trustees and Officers section of the Funds' SAI, the following table replaces the corresponding "Other Accounts Managed" table in its entirety:

Other Registered

Investment

Companies

Other Pooled

Investment

Vehicles


Other

Accounts


Ashwin Alankar

Number of Other Accounts Managed 4  1  14(1)
Assets in Other Accounts Managed $330.83M  $22.98M  $1,939.82M 

Faizan Baig

Number of Other Accounts Managed 1  None  2 
Assets in Other Accounts Managed $54.93M  None  $172.01M 

Jenna Barnard

Number of Other Accounts Managed None  5  1 
Assets in Other Accounts Managed None  $3,288.31M  $225.02M 

Craig Kempler

Number of Other Accounts Managed None  None  2 
Assets in Other Accounts Managed None  None  $65.95M 

Michael Keough

Number of Other Accounts Managed 6  13  17 
Assets in Other Accounts Managed $39,748.44M  $11,772.53M  $5,419.38M 

John Kerschner

Number of Other Accounts Managed 6  3  3 
Assets in Other Accounts Managed

$29,695.34M 

$535.42M  $160.40M 

Other Registered

Investment

Companies

Other Pooled
Investment

Vehicles

Other

Accounts

John Lloyd

Number of Other Accounts Managed 2  8  31 
Assets in Other Accounts Managed $753.05M  $2,974.21M  $2,614.73M 

Ben Lofthouse

Number of Other Accounts Managed 2  1  2 
Assets in Other Accounts Managed $6,350.34M  $892.92M  $172.01M 

Seth Meyer

Number of Other Accounts Managed None  3  1 
Assets in Other Accounts Managed None  $535.42M  $0.11M 

Brent Olson

Number of Other Accounts Managed None  4  5 
Assets in Other Accounts Managed None  $1,160.12M  $766.62M 

Kevin Preloger(2)

Number of Other Accounts Managed 2(3) 2  3 
Assets in Other Accounts Managed $199.26M  $168.52M  $106.60M 

Tom Ross

Number of Other Accounts Managed None  5  8 
Assets in Other Accounts Managed None  $1,562.70M  $1,762.19M 

Brad Smith

Number of Other Accounts Managed 1  7  8 
Assets in Other Accounts Managed $25.05M  $1,499.71M  $4,362.84M 

Justin Tugman

Number of Other Accounts Managed 2(3) 2  5 
Assets in Other Accounts Managed $199.26M  $168.52M  $172.55M 

Nicholas Ware

Number of Other Accounts Managed None  4  1 
Assets in Other Accounts Managed None  $3,009.39M  $225.02M 

Greg Wilensky

Number of Other Accounts Managed 5  9  12 
Assets in Other Accounts Managed $39,723.39M  $11,374.41M  $3,869.25M 
(1)

One account included in the total, consisting of $761.30M of the total assets in the category, has a performance-based advisory fee.

(2)

Kevin Preloger retired from the Adviser on December 31, 2025.

(3)

One account included in the total, consisting of $114.47M of the total assets in the category, has a performance-based advisory fee.

2

Please retain this Supplement with your records.

Janus Investment Fund

Janus Henderson Global Allocation Fund - Conservative

Janus Henderson Global Allocation Fund - Growth

Janus Henderson Global Allocation Fund - Moderate

(the "Funds")

Supplement dated March 4, 2026

to Currently Effective Statement of Additional Information

Effective immediately, the statement of additional information ("SAI") for the Funds is updated as follows:

1.

Under "Janus Henderson Portfolio Management" in the Trustees and Officers section of the Funds' SAI, the following table replaces the corresponding "Other Accounts Managed" table in its entirety:

Other Registered

Investment

Companies

Other Pooled

Investment

Vehicles


Other

Accounts


Ashwin Alankar

Number of Other Accounts Managed 2  1  14(1)
Assets in Other Accounts Managed $789.30M  $22.98M  $1,939.82M 

Nick Harper

Number of Other Accounts Managed None  1  4 
Assets in Other Accounts Managed None  $242.16M  $3.76M 
(1)

One account included in the total, consisting of $761.30M of the total assets in the category, has a performance-based advisory fee.

Please retain this Supplement with your records.

Janus Investment Fund

Janus Henderson Small Cap Value Fund

Class L Shares

Supplement dated March 4, 2026

to Currently Effective Statement of Additional Information

Effective immediately, the statement of additional information ("SAI") for Janus Henderson Small Cap Value Fund (the "Fund") is updated as follows:

1.

Under "Janus Henderson Portfolio Management" in the Trustees and Officers section of the Fund's SAI, the following table replaces the corresponding "Other Accounts Managed" table in its entirety:

Other Registered

Investment

Companies

Other Pooled

Investment

Vehicles


Other

Accounts


Craig Kempler

Number of Other Accounts Managed None  None  2 
Assets in Other Accounts Managed None  None  $65.95M 

Justin Tugman

Number of Other Accounts Managed 4(1) 2  5 
Assets in Other Accounts Managed $2,268.57M  $168.52M  $172.55M 
(1)

Three accounts included in the total, consisting of $2,183.78M of the total assets in the category, have performance-based advisory fees.

Please retain this Supplement with your records.

Janus Investment Fund published this content on March 04, 2026, and is solely responsible for the information contained herein. Distributed via EDGAR on March 04, 2026 at 11:03 UTC. If you believe the information included in the content is inaccurate or outdated and requires editing or removal, please contact us at [email protected]