Failure to Satisfy Listing Rule (Form 8-K)

Item 3.01. Notice of Delisting or Failure to Satisfy a Continued Listing Rule or Standard; Transfer of Listing.

On June 9, 2025, Predictive Oncology Inc. (the "Company") received a letter (the "Notice) from the Listing Qualifications Department (the "Staff") of The Nasdaq Stock Market LLC ("Nasdaq") notifying the Company that because the Company had not regained compliance with the minimum $2,500,000 stockholders' equity requirement for continued listing on The Nasdaq Capital Market as set forth in Nasdaq Listing Rule 5550(b)(1) (the "Stockholders' Equity Requirement"), the Staff has determined to delist the Company's securities from The Nasdaq Capital Market. The Company was unable to complete its previously submitted plan of compliance within the 180-day extension period provided under the Listing Rules.

Unless the Company requested an appeal of the Staff's determination by June 16, 2025, trading of the Company's common stock would be suspended at the opening of business on June 18, 2025 and a Form 25-NSE would be filed with the Securities and Exchange Commission ("SEC"), which would remove the Company's securities from listing and registration on the Nasdaq.

The Company has submitted a hearing request to Nasdaq's Hearings Panel (the "Panel"), and the Panel has received the Company's request, to appeal the Staff's determination, which will stay the suspension of the Company's securities and the filing of the Form 25-NSE pending the Panel's decision. The Panel has discretion to take actions as prescribed by Nasdaq Listing Rule 5815(c)(1) including, but not limited to, granting an exception to the continued listing standards for a period not to exceed 180 days from the date of the Notice. However, there can be no assurance that the Company's request to continue listing will be accepted or that if it is, the Company will be able to maintain compliance with requirements for its continued listing on The Nasdaq Capital Market.

The Company will continue to evaluate all available options to regain compliance with the listing requirements.