NIST - National Institute of Standards and Technology

09/24/2026 | Press release | Distributed by Public on 09/25/2026 03:24

NISTCHO Cell Culture and Fed-Batch Bioreactor Protocols

Published
September 24, 2026

Author(s)

Ioannis Karageorgos, William O'Dell, Vamsi Bolla, Hussain Dahodwala, Hussain Dahodwala, Zvi Kelman, John Marino

Abstract

The NISTCHO cell system (RM 8675) provides a standardized Chinese hamster ovary (CHO) cell culture platform for studies of recombinant monoclonal antibody production and bioprocess characterization. Reproducible recovery, expansion, cultivation, and monitoring of NISTCHO cells are important for generating materials with controlled and documented process histories. This Special Publication describes an integrated upstream workflow beginning with recovery of cryopreserved NISTCHO cells and continuing through automated cell concentration and viability measurement, preparation of chemically defined feed media, seed-train expansion, and fed-batch cultivation in a single-use stirred-tank bioreactor. The procedures define operating conditions for temperature, agitation, dissolved oxygen, pH, feeding, glucose supplementation, antifoam addition, sampling, harvest, and initial clarification. A representative 14-day process profile is included to illustrate typical NISTCHO culture behavior. The procedures are intended to facilitate reproducible implementation of the NISTCHO upstream process and generation of cNISTmAb-containing harvest material for downstream processing and analytical studies.
Citation
Special Publication (NIST SP) - 1200-32
Report Number
1200-32
Pub Type
NIST Pubs

Keywords

Bioprocessing, cNISTmAb, Chinese Hamster Ovary Cells, Cell Culture, Fed-batch, Monoclonal Antibody, NISTCHO.

Citation

Karageorgos, I. , O'Dell, W. , Bolla, V. , Dahodwala, H. , Dahodwala, H. , Kelman, Z. and Marino, J. (2026), NISTCHO Cell Culture and Fed-Batch Bioreactor Protocols, Special Publication (NIST SP), National Institute of Standards and Technology, Gaithersburg, MD, [online], https://doi.org/10.6028/NIST.SP.1200-32, https://tsapps.nist.gov/publication/get_pdf.cfm?pub_id=962965 (Accessed September 25, 2026)
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NIST - National Institute of Standards and Technology published this content on September 24, 2026, and is solely responsible for the information contained herein. Distributed via Public Technologies (PUBT), unedited and unaltered, on September 25, 2026 at 09:24 UTC. If you believe the information included in the content is inaccurate or outdated and requires editing or removal, please contact us at [email protected]