Material Event (Form 8-K)

Item 8.01 Other Events.

As previously reported, on May 31, 2024, Ainos, Inc. (the "Company") entered into an At The Market Offering Agreement (the "Agreement") with H.C. Wainwright & Co., LLC as placement agent (the "Placement Agent") to offer and sell shares of its common stock, par value $0.01 per share ("Common Stock"), having an aggregate offering price of up to $1,353,197 from time to time through the Placement Agent. On July 11, 2024, the Company filed a prospectus supplement under the Agreement to increase the aggregate offering price of Common Stock to be offered and sold under the Agreement to $1,840,350. Prior to the date hereof, the Company sold shares of Common Stock having an aggregate sales price of approximately $1,840,337 under the Agreement. On September 5, 2025, the Company filed a prospectus supplement under the Agreement for an aggregate of $874,496 of additional shares of Common Stock.

A copy of the legal opinion of Lucosky Brookman LLP, relating to the validity of the shares in connection with the Agreement is filed as Exhibit 5.1 to this Current Report on Form 8-K and is incorporated herein by reference.