Additional Proxy Soliciting Materials (Form DEFA14A)

UNITED STATES

SECURITIES AND EXCHANGE COMMISSION

WASHINGTON, D.C. 20549

SCHEDULE 14A

Proxy Statement Pursuant to Section 14(a) of the

Securities Exchange Act of 1934

(Amendment No. )

Filed by the Registrant ☒

Filed by a Party other than the Registrant ☐

Check the appropriate box:

Janus Investment Fund

(Name of Registrant as Specified in Its Charters)

(Name of Person(s) Filing Proxy Statement, if other than the Registrant)

Payment of Filing Fee (Check all boxes that apply):

40 Act Proxy Video - FUND FOCUS

ADVISOR AUDIENCE

Hi, I'm Ali Dibadj, CEO of Janus Henderson.

Thank you for allowing me to take a couple minutes of your time to update you on our company and where we need your help, please.

As you may know, Janus Henderson has agreed to become a privately-owned company, which, to be clear, is a change of ownership in the company but not much else.

To allow Janus Henderson to continue as the investment adviser on your clients' funds after the process of going private ends, fund shareholders (including myself) are being asked to approve a new investment advisory agreement. That's the reason why Alliance Advisors, our selected proxy solicitor, has been in touch by all those mailings, emails, text messages, and phone calls asking them to vote.

We apologize that the contact from them has been so frequent-I've gotten lots of contact too-but, we actually have an obligation to ask fund shareholders to vote and return their proxy as soon as possible. Doing so will stop further follow-up communications and allow us to continue serving as their investment adviser.

We want you to know that there will be no change in the way we manage our funds, the fee rate charged, or in how you do business with Janus Henderson as a result of us becoming a privately owned company. The investment team who currently manage your clients' investments will continue to do so under the existing investment objectives and policies. Continuing to serve our 75 million investors well is our number one priority.

We've created a number of convenient options for fund shareholders to vote ahead of the May 18^th virtual shareholder meeting - that can be done by phone, by mail, through a text message link, or by using an app that includes all of their accounts in one place. Personally, I just called the phone number shown on the screen and it took me less than two minutes to vote everything. They can visit the website for more information. [show those but keep the phone number on].

At Janus Henderson, we pride ourselves on clients coming first-always, and if you have any questions whatsoever, please reach out to your usual contact and we'd be more than happy to help.

As always, we thank you very much for the trust and confidence you have placed in Janus Henderson.

***ENDS***

Janus Henderson^® and any other trademarks used herein are trademarks of Janus Henderson Group plc or one of its subsidiaries. ^© Janus Henderson Group plc.

Certain statements in this communication not based on historical facts are "forward-looking statements" within the meaning of the federal securities laws. Such forward-looking statements involve known and unknown risks and uncertainties that are difficult to predict and could cause our actual results, performance or achievements to differ materially from those discussed. These include statements as to funds' future expectations, beliefs, plans, strategies, objectives, events, conditions, financial performance, prospects or future events, including with respect to the timing and anticipated benefits of pending and recently completed transactions and strategic partnerships, and expectations regarding opportunities that align with our strategy. In some cases, forward-looking statements can be identified by the use of words such as "may," "could," "expect," "intend," "plan," "seek," "anticipate," "believe," "estimate," "predict," "potential," "continue," "likely," "will," "would," and similar words and phrases. Forward-looking statements are necessarily based on estimates and assumptions that, while considered reasonable by, as applicable, the funds and their respective management, are inherently uncertain. Accordingly, you should not place undue reliance on forward-looking statements, which speak only as of the date they are made and are not guarantees of future performance. The funds do not undertake any obligation to publicly update or revise these forward-looking statements.

Various risks, uncertainties, assumptions and factors that could cause the funds' future results to differ materially from those expressed by the forward-looking statements included in this communication include, but are not limited to, the impact of any alternative proposal, Janus Henderson's ability to obtain the applicable regulatory, shareholder and other approvals required to consummate the proposed transaction and the timing of the closing of the proposed transaction, that the funds may be adversely affected by other economic, business, and/or competitive factors, volatility or disruption in financial markets and other risks, uncertainties, assumptions, and factors discussed in each funds' current prospectus.

In connection with the solicitation of proxies from fund shareholders, the funds filed definitive proxy statements with the SEC on March 2, 2026. The funds may also file other documents with the SEC regarding the solicitation of proxies from fund shareholders, including amendments to the proxy statements. This communication is not a substitute for any proxy statement or any other document that may be filed by the funds with the SEC. INVESTORS AND SECURITY HOLDERS OF THE FUNDS ARE URGED TO READ THE PROXY STATEMENT AND ANY OTHER RELEVANT DOCUMENTS FILED WITH THE SEC CAREFULLY AND IN THEIR ENTIRETY, BECAUSE THEY CONTAIN OR WILL CONTAIN IMPORTANT INFORMATION. Investors and security holders will be able to obtain the proxy statement and other documents that are filed with the SEC by the funds free of charge from the SEC's website at https://www.sec.gov.

The funds and their manager, trustees and executive officers may be deemed to be participants in the solicitation of proxies from fund shareholders in connection with the proposed transaction. Information about the trustees and executive officers of the funds and their ownership of the funds is contained in each fund's current statement of additional information. Additional information regarding the persons who may, under the rules of the SEC, be deemed participants in the solicitation of the shareholders of the funds, including a description of their direct or indirect interests, by security holdings or otherwise, will be included in the funds' proxy statements. Free copies of the funds' proxy statements and free copies of the other SEC filings to which reference is made in this paragraph may be obtained from the SEC's website at https://www.sec.gov

40 Act Proxy Video - FUND FOCUS

DIRECT RETAIL AUDIENCE

Hi, I'm Ali Dibadj, CEO of Janus Henderson.

Thank you for allowing me to take a couple minutes of your time to update you on our company and where we need your help, please.

As you may know, Janus Henderson has agreed to become a privately-owned company, which, to be clear, is a change of ownership in the company but not much else.

To allow Janus Henderson to continue as the investment adviser on your funds after the process of going private ends, we fund shareholders (because I am one too) are being asked to approve a new investment advisory agreement. That's the reason why Alliance Advisors, our selected proxy solicitor, has been in touch by all those mailings, emails, text messages, and phone calls asking you to vote.

We apologize that the contact from them has been so frequent-I've gotten lots of contact too-but, we actually have an obligation to ask fund shareholders to vote and return their proxy as soon as possible. Doing so will stop further follow-up communications and allow us to continue serving as your investment adviser.

We want you to know that there will be no change in the way we manage our funds, the fee rate charged, or in how you do business with Janus Henderson as a result of us becoming a privately owned company. The investment team who currently manage your investments will continue to do so under the existing investment objectives and policies. Continuing to serve our 75 million investors well is our number one priority.

We've created a number of convenient options for fund shareholders to vote ahead of the May 18^th virtual shareholder meeting - that can be done by phone, by mail, through a text message link, or by using an app that includes all of your accounts in one place. Personally, I just called the phone number shown on the screen and it took me less than two minutes to vote everything. Please visit the website for more information. [show those but keep the phone number on].

At Janus Henderson, we pride ourselves on clients coming first-always, and if you have any questions whatsoever, please reach out to us and we'd be more than happy to help.

As always, we thank you very much for the trust and confidence you have placed in Janus Henderson.

***ENDS***

Janus Henderson^® and any other trademarks used herein are trademarks of Janus Henderson Group plc or one of its subsidiaries. ^© Janus Henderson Group plc.

Certain statements in this communication not based on historical facts are "forward-looking statements" within the meaning of the federal securities laws. Such forward-looking statements involve known and unknown risks and uncertainties that are difficult to predict and could cause our actual results, performance or achievements to differ materially from those discussed. These include statements as to funds' future expectations, beliefs, plans, strategies, objectives, events, conditions, financial performance, prospects or future events, including with respect to the timing and anticipated benefits of pending and recently completed transactions and strategic partnerships, and expectations regarding opportunities that align with our strategy. In some cases, forward-looking statements can be identified by the use of words such as "may," "could," "expect," "intend," "plan," "seek," "anticipate," "believe," "estimate," "predict," "potential," "continue," "likely," "will," "would," and similar words and phrases. Forward-looking statements are necessarily based on estimates and assumptions that, while considered reasonable by, as applicable, the funds and their respective management, are inherently uncertain. Accordingly, you should not place undue reliance on forward-looking statements, which speak only as of the date they are made and are not guarantees of future performance. The funds do not undertake any obligation to publicly update or revise these forward-looking statements.

Various risks, uncertainties, assumptions and factors that could cause the funds' future results to differ materially from those expressed by the forward-looking statements included in this communication include, but are not limited to, the impact of any alternative proposal, Janus Henderson's ability to obtain the applicable regulatory, shareholder and other approvals required to consummate the proposed transaction and the timing of the closing of the proposed transaction, that the funds may be adversely affected by other economic, business, and/or competitive factors, volatility or disruption in financial markets and other risks, uncertainties, assumptions, and factors discussed in each funds' current prospectus.

In connection with the solicitation of proxies from fund shareholders, the funds filed definitive proxy statements with the SEC on March 2, 2026. The funds may also file other documents with the SEC regarding the solicitation of proxies from fund shareholders, including amendments to the proxy statements. This communication is not a substitute for any proxy statement or any other document that may be filed by the funds with the SEC. INVESTORS AND SECURITY HOLDERS OF THE FUNDS ARE URGED TO READ THE PROXY STATEMENT AND ANY OTHER RELEVANT DOCUMENTS FILED WITH THE SEC CAREFULLY AND IN THEIR ENTIRETY, BECAUSE THEY CONTAIN OR WILL CONTAIN IMPORTANT INFORMATION. Investors and security holders will be able to obtain the proxy statement and other documents that are filed with the SEC by the funds free of charge from the SEC's website at https://www.sec.gov.

The funds and their manager, trustees and executive officers may be deemed to be participants in the solicitation of proxies from fund shareholders in connection with the proposed transaction. Information about the trustees and executive officers of the funds and their ownership of the funds is contained in each fund's current statement of additional information. Additional information regarding the persons who may, under the rules of the SEC, be deemed participants in the solicitation of the shareholders of the funds, including a description of their direct or indirect interests, by security holdings or otherwise, will be included in the funds' proxy statements. Free copies of the funds' proxy statements and free copies of the other SEC filings to which reference is made in this paragraph may be obtained from the SEC's website at https://www.sec.gov

Email and Broadcast Message

Subject Line: Time-Sensitive: You only have days left to vote

Preheader: The Joint Special Meeting of Shareholders is on May 18^th, 2026 and is the deadline for you to submit your vote.

Copy:

Hello,

As a direct investor in our mutual funds, we are reaching out to notify you that the Joint Special Meeting of Shareholders is on May 18^th, 2026 and the deadline to submit your proxy vote is rapidly approaching. According to our most recent records, your voting instructions have not yet been received.

The Board of Trustees of your mutual fund unanimously recommends that shareholders vote "FOR" all proposals, please review the proxy materials at Janus Investment Fund's SEC Filings.

Option 1: Your vote is very important, please vote today!

Option 2: Exercise your individual rights as a shareholder

How to cast your vote:

Questions? Visit the Janus Henderson Proxy Voting Site

Button: View Now

Secure Message Center (Plain Text)

Hello,

As a direct investor in our mutual funds, we are reaching out to notify you that the Joint Special Meeting of Shareholders is on May 18^th, 2026 and the deadline to submit your proxy vote is rapidly approaching. According to our most recent records, your voting instructions have not yet been received.

The Board of Trustees of your mutual fund unanimously recommends that shareholders vote "FOR" all proposals, please review the proxy materials at Janus Investment Fund's SEC Filings.

Your vote is very important, please vote today!

How to cast your vote:

Questions? Visit the Janus Henderson Proxy Voting Site

View Now

Call Flow Summary and Scripting

Inbound Call Flow

Callback Offer

Thank you for your patience. To receive a callback and retain your place in line, please press 1 now.

Introduction Script

Thank you for your patience. For faster service, please listen carefully to the following options.

Press 1 to speak with our Digital Assistant for assistance with voting instructions or frequently asked questions.

Press 2 to continue holding for the next available proxy voting specialist.

Digital Assistant Scripting

Call greeting: "Hello, thank you for calling the Janus Henderson ETF/Mutual Fund shareholders information line. My name is _______ and I am a Digital Agent. This call is being recorded for quality assurance. How may I assist you today?"

Responding to Caller Needs:

Identity Collection:

Account Lookup:

Voting Assistance:

Vote Submission and Confirmation:

Special Cases: