Antibiotic pollution could accelerate amphibian decline by turning a potential solution into a threat

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Frogs, toads, salamanders and other amphibians are disappearing as fast as-or faster than-any other class of animals around the world, succumbing to a variety of threats, like emerging infectious diseases. According to new research from the University of Wisconsin-Madison, one promising way to protect frogs from a particularly deadly fungal disease may be less useful than previously thought thanks to waterways polluted by a treatment for infections: antibiotic drugs.

The fungus known as Bd, Batrachochytrium dendrobatidis, is responsible for a skin disease that has contributed to declines in amphibian diversity in many parts of the world.

Bd doesn't have free reign, though, even in ponds where it causes many infections. It has enemies down on its own microscopic scale, microbes that are competing hard in their own ecological niches. Scientists have hypothesized that chemical mixtures produced by those microbes could act like a vaccine against Bd.

"In your own microbiota - in your gut, on your skin - you have different species of bacteria living in a particular area," says Jessica Hua, a UW-Madison professor of forest and wildlife ecology who studies how ecosystem disturbances like pollution affect ecology and evolution. "When there's high competition, particular microbial species like these generate compounds that inhibit other bacteria and microorganisms from succeeding."

Those chemical defenses are a reaction to the bacteria's environment. But competition with fungi like Bd is just one aspect of the environment to which microbes need to adapt. More and more, bacteria have to contend with water contaminated by antibiotic drugs-a pollutant growing more common in waterways affected by runoff from farms, zoos and wastewater treatment plants.

Hua and her colleagues wanted to find out whether, a common bacterium, Pseudomonas aeruginosa, would retain its Bd-fighting abilities while also fending off assaults from antibiotic pollution.

"Depending on the environment they live in, those bacteria may react by changing that anti-pathogenic cocktail of compounds they produce," Hua says. "If they're really comfortable and stress-free, they might put their resources into growth or some other need. If they have to adapt to a new or unusual problem, like pollutants, that can come at a cost that shifts the cell physiology of the microorganism."

Hua's lab, with collaborators at Binghamton University and New Mexico State University, exposed Bd to the chemical cocktail produced by strains of Pseudomonas that had adapted to water contaminated by antibiotic drugs and to the chemicals made by Pseudomonas that hadn't had to figure out how to survive alongside antibiotics. They also raised tadpoles in water with Bd and the chemical cocktail from bacteria that had and had not been forced to adapt to antibiotics.

The chemical mix from Pseudomonas that hadn't been exposed to antibiotics was indeed bad news for Bd, reducing the fungi's growth rate significantly in lower concentrations and entirely in large doses. Tadpoles benefitted, too. They were less likely to be infected with Bd while living in water with the bacteria that hadn't developed tolerance to antibiotics.

However, chemicals from Pseudomonas that had adapted to antibiotic pollution actually helped Bd. The troublesome fungi grew faster in water with the products of antibiotic-tolerant Pseudomonas, and tadpoles in that mix were six times more likely to be infected with Bd than those paired with chemical from bacteria unbothered by antibiotics.

The researchers published their findings recently in the journal Scientific Reports.

"This shows us that if we're going to develop some sort of treatment for an emerging disease this way, we have to consider the history of the microbes we're looking at," says Hua, whose work is supported by the National Science Foundation. "The traits, the effect on amphibians, you're looking for could very easily change-and here we've seen them change from help to harm-especially in light of increasing pollution."

To further complicate matters, the scientists repeated their experiments with strains of Pseudomonas that clump together in colonies called biofilms, instead of strains that float free in water. The results flipped entirely. The chemical cocktail from biofilm-forming Pseudomonas that had not encountered antibiotics was worse for the tadpoles and better for Bd. The antibiotic-tolerant, biofilm-forming bacteria inhibited Bd growth and kept tadpoles healthier longer.

"It was surprising to see just how opposite it was with just this change in Pseudomonas behavior," Hua says. "There are so many considerations. Ignoring them, we might actually do more harm than good."

This research was supported in part by grants from the National Science Foundation (2137424 and 2314625).