Failure to Satisfy Listing Rule (Form 8-K)

Item 3.01. Notice of Delisting or Failure to Satisfy a Continued Listing Rule or Standard; Transfer of Listing

As previously reported, The Nasdaq Stock Market LLC's ("Nasdaq") Listing Qualifications Staff (the "Staff") notified Novo Integrated Sciences, Inc. (the "Company") that it was in violation of the minimum bid price requirement of Nasdaq Listing Rule 5550(a)(2). Also as previously disclosed, on November 4, 2024, the Company received a letter from Nasdaq indicating that the Company's common stock would be delisted from Nasdaq and trading in the Company's common stock would be suspended at the open of trading on November 6, 2024. Beginning at the open of trading on November 6, 2024, the Company's common stock began trading on the OTC Market under the symbol "NVOS."

On April 28, 2025, Nasdaq notified the Company that it would file a Form 25 with the Securities and Exchange Commission to complete the delisting of the Company's common stock. As of the filing time of this Current Report on Form 8-K, the Form 25 has not yet been filed by Nasdaq. The delisting will be effective 10 days after the Form 25 is filed. The Company's common stock will continue to be quoted on the OTC Market following filing and effectiveness of the Form 25.